Increased Cloning Efficiency by Temperature-Cycle Ligation

Increased cloning efficiency by temperature-cycle ligation.

Molecular cloning is a crucial, and often speed-limiting, step in many standard procedures of molecular biology. Ligation of cohesive DNA ends is normally carried out at 12–16 C to ensure a good balance between enzyme activity and stability of annealed DNA overhangs. Low temperatures generally reduce ligase activity, whereas too high temperatures may reduce cloning efficiencies by melting annea...

Low Temperature Heating Systems Increased Energy Efficiency and Improved Comfort Low Temperature Heating Systems Increased Energy Efficiency and Improved Comfort

Improvement of Efficiency of Coal-Fired Steam Power Plant by Reducing Heat Rejection Temperature at Condenser Using Kalina Cycle

This paper proposes an approach for improving the plant efficiency by reducing the heat rejection temperature of power cycle using Kalina Cycle System 11 (KCS11) which is integrated at the steam condenser of a 500 MWe SubC (subcritical) coal-fired power plant. It is modelled by using power plant simulation software ‘Cycle Tempo’ at different plant operating conditions. Results show t...

Recombinant expressed vector pET32a (+) S constructed by ligation independent cloning.

The aim of this work was to develop a new method for constructing vectors, named ligation-independent cloning (LIC) method. We constructed the S label expression vector and recombinant pET32a (+) S-phoN2 by LIC. The recombinant proteins were expressed in E. coli at a high level, and then the specificity of the recombinant proteins was identified by western blot. The target band was detected by ...

High-efficiency T-vector cloning of PCR products by forced A tagging and post-ligation restriction enzyme digestion.